Hal Robson-Kanu of West Bromwich Albion and Shkodran Mustafi of Arsenal battle for the ball during the Premier League match between Arsenal and West Bromwich Albion at Emirates Stadium on September 25, 2017 in London, England.
It means Mustafi isn't expected to be available before the November global break and will therefore miss Arsenal's next seven games including Premier League fixtures against Watford, Everton, Swansea and Manchester City.
Kolasinac only managed one half while in action for Bosnia-Herzegovina against Belgium due to a hip injury, while Koscielny is set for a late fitness test on an Achilles injury.
"Kolasinac is a minor problem, a hip problem".
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Both deadlines have been included in a formal demand sent to the Catalan government. The legal validity of the declaration was unclear.
Wenger explained: "Koscielny has a definite final test tomorrow".
"We've lost Mustafi for about four to six weeks", said Gunners manager Arsene Wenger.
"I will decide what I have to do when Koscielny has his test tomorrow".
Wenger added: "The player I expected to lose the least during the break was Mustafi". We will check him today.
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'I didn't expect him to play and Germany had already qualified.
But Watford are yet to win any of their four home games in any competition this season, while Arsenal - 7/10 (1.70) to win the match outright - have won four on the spin ahead of their Saturday teatime trip to Vicarage Road, where they won 3-1 last season.
Wenger said: "We will assess Sanchez when he comes back, I watched the Brazil game and he got some special treatment in that game". "I am more concerned that yesterday afternoon I watched the whole game Brazil against Chile to see how hard the game was and I must say he got some special treatment in this game".
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The hack included information on the F-35 Joint Strike Fighter, C130 Hercules aircraft and the P-8 Poseidon surveillance aircraft. Mr Clarke said the hack was "extensive and extreme" and took advantage of "sloppy" security at the contractor.